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polyclonal rabbit anti mouse ldha  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit anti mouse ldha
    Polyclonal Rabbit Anti Mouse Ldha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/LDHA+Antibody/pm40981418-417-27-35
    Average 96 stars, based on 1106 article reviews
    polyclonal rabbit anti mouse ldha - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: UCP2 Deficiency Increases Colon Tumorigenesis by Promoting Lipid Synthesis and Depleting NADPH for Antioxidant Defenses
    Article Snippet: Rabbit polyclonal Anti-LDHA , Cell Signaling , Cat# 2012S; RRID: AB_2137173.

    Article Title: SUPPRESSING MITOCHONDRIAL RESPIRATION IS CRITICAL FOR HYPOXIA TOLERANCE IN THE FETAL GROWTH PLATE
    Article Snippet: Rabbit polyclonal anti LDHA , Cell Signaling Technology , Cat# 2012, RRID:AB_2137173.

    Article Title: Interferon Gamma Induces Reversible Metabolic Reprogramming of M1 Macrophages to Sustain Cell Viability and Pro-Inflammatory Activity
    Article Snippet: Rabbit polyclonal anti-LDHa , Cell Signaling Technology , Cat# 2012.

    Article Title: Carbon source availability drives nutrient utilization in CD8 + T cells
    Article Snippet: Rabbit polyclonal anti-Ldha , Cell Signaling , #2012.

    Article Title: Lactate Dehydrogenase A Governs Cardiac Hypertrophic Growth in Response to Hemodynamic Stress
    Article Snippet: Rabbit polyclonal anti-LDHA , Cell Signaling Technology , Cat# 2012; RRID:AB_2137173.

    Staining:

    Article Title: Astrocytic lactate dehydrogenase A regulates neuronal excitability and depressive-like behaviors through lactate homeostasis in mice.
    Article Snippet: 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 0190), DL-2amino-5-phosphonopentanoic acid (DL-AP5, 0105), and SR-95531 (1262) were purchased from Tocris Bioscience. .. The following primary antibodieswereused: rabbit polyclonal anti-LDHA (Cell Signaling Technology, 2012S; 1:1000 for blotting);mousemonoclonal anti-LDHB (Santa Cruz Biotechnology, sc-100775, 431.1; 1:1000 for blotting); mousemonoclonal anti-α-Tubulin (SantaCruzBiotechnology, sc-5286, B-7; 1:1000 for blotting); mouse monoclonal anti-GFAP (Millipore, MAB360, GA5; 1:1000 for blotting); mouse monoclonal anti-Tuj1 (Cell Signaling Technology, 4466, TU-20; 1:1000 for blotting); mouse monoclonal anti-NeuN (Millipore, MAB377, A60; 1:200 for immunofluorescent staining); rabbit monoclonal anti-S100ß (abcam, ab52642, EP1576Y; 1:200 for immunofluorescent staining). ..

    Article Title: Astrocytic lactate dehydrogenase A regulates neuronal excitability and depressive-like behaviors through lactate homeostasis in mice
    Article Snippet: 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 0190), DL-2-amino-5-phosphonopentanoic acid (DL-AP5, 0105), and SR-95531 (1262) were purchased from Tocris Bioscience. .. The following primary antibodies were used: rabbit polyclonal anti-LDHA (Cell Signaling Technology, 2012S; 1:1000 for blotting); mouse monoclonal anti-LDHB (Santa Cruz Biotechnology, sc-100775, 431.1; 1:1000 for blotting); mouse monoclonal anti-α-Tubulin (Santa Cruz Biotechnology, sc-5286, B-7; 1:1000 for blotting); mouse monoclonal anti-GFAP (Millipore, MAB360, GA5; 1:1000 for blotting); mouse monoclonal anti-Tuj1 (Cell Signaling Technology, 4466, TU-20; 1:1000 for blotting); mouse monoclonal anti-NeuN (Millipore, MAB377, A60; 1:200 for immunofluorescent staining); rabbit monoclonal anti-S100ß (abcam, ab52642, EP1576Y; 1:200 for immunofluorescent staining). ..



    Similar Products

    rabbit anti-ldha polyclonal antibody  (Thermo Fisher)
    90
    Thermo Fisher rabbit anti-ldha polyclonal antibody
    <t>LDHA</t> expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired
    Rabbit Anti Ldha Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/lactate+dehydrogease+a+ldha+pa5+23036/pmc11110387-98-23-28
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    polyclonal rabbit anti mouse ldha  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc polyclonal rabbit anti mouse ldha
    <t>LDHA</t> expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired
    Polyclonal Rabbit Anti Mouse Ldha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/LDHA+Antibody/pm40981418-417-27-35
    Average 96 stars, based on 1 article reviews
    polyclonal rabbit anti mouse ldha - by Bioz Stars, 2026-09
    96/100 stars
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    anti ldha specific rabbit polyclonal  (Proteintech)
    96
    Proteintech anti ldha specific rabbit polyclonal
    <t>LDHA</t> expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired
    Anti Ldha Specific Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/LDHA-Specific+Antibody/pmc12116918-317-85-88
    Average 96 stars, based on 1 article reviews
    anti ldha specific rabbit polyclonal - by Bioz Stars, 2026-09
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    antibody rabbit polyclonal anti-ldha  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology antibody rabbit polyclonal anti-ldha
    <t>LDHA</t> expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired
    Antibody Rabbit Polyclonal Anti Ldha, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/rabbit+polyclonal+anti+ldha/pm40132584-304-71-85
    Average 90 stars, based on 1 article reviews
    antibody rabbit polyclonal anti-ldha - by Bioz Stars, 2026-09
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    rabbit polyclonal anti-phospho-ldha  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology rabbit polyclonal anti-phospho-ldha
    <t>LDHA</t> expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired
    Rabbit Polyclonal Anti Phospho Ldha, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/rabbit+polyclonal+anti+ldha/pmc11584876__41467_2024_54377_MOESM7_ESM-83-76-80
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    rabbit polyclonal anti ldha antibody  (Boster Bio)
    93
    Boster Bio rabbit polyclonal anti ldha antibody
    The <t>LDHA</t> expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Anti Ldha Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/Anti-LDHA+Antibody+Picoband/pmc11581964-115-4-10
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    rabbit polyclonal anti ldha antibody - by Bioz Stars, 2026-09
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    rabbit polyclonal antibody  (Proteintech)
    96
    Proteintech rabbit polyclonal antibody
    The <t>LDHA</t> expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/LDHA-Specific+Antibody/pm39173867-59-13-88
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    rabbit polyclonal anti ldha  (Proteintech)
    96
    Proteintech rabbit polyclonal anti ldha
    The <t>LDHA</t> expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Anti Ldha, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/LDHA+Antibody/pmc11372217__41467_2024_51882_MOESM6_ESM-50-64-67
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal anti ldha - by Bioz Stars, 2026-09
    96/100 stars
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    rabbit polyclonal antildha antibody  (Boster Bio)
    93
    Boster Bio rabbit polyclonal antildha antibody
    The <t>LDHA</t> expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.
    Rabbit Polyclonal Antildha Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ldha/Anti-LDHA+Antibody+Picoband/pm39582531-77-4-10
    Average 93 stars, based on 1 article reviews
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    Image Search Results


    LDHA expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired

    Journal: Cell & Bioscience

    Article Title: Targeting miR-181a/b in retinitis pigmentosa: implications for disease progression and therapy

    doi: 10.1186/s13578-024-01243-3

    Figure Lengend Snippet: LDHA expression decreases with downregulation of miR-181a/b in the RPE of Pde6β H620Q mice at PW6. A Illustration of the proposed hypothesis: miR-181a/b downregulation makes RPE cells healthier and decreases the aerobic glycolysis in those cells. B – C qPCR analysis of the expression of key regulators of aerobic glycolysis in the RPE cells of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at ( B ) PW6, and C PW10. N ≥ 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. D – F Western Blotting analysis in ( D ) reveals decreased levels of LDHA protein in the RPE cells of Pde6β H620Q miR-181a/b-1 −/− versus Pde6β H620Q miR-181a/b-1 +/+ . Data are normalized to β-actin. N = 3 mice/genotype. Please note that all compared bands are from the same blots. LDHA bands are quantified in ( E ) and PKM2 bands in ( F ). N = 6 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. G – K Analysis of the expression of key regulators of aerobic glycolysis at ( G ) mRNA and H – K protein levels in the NR of Pde6β H620Q miR-181a/b-1 +/+ and Pde6β H620Q miR-181a/b-1 −/− mice at PW6. Data are presented as mean ± SEM for N ≥ 3 mice/genotype and Student’s t-test, unpaired in ( G ). Please note that all compared bands are from the same blots in ( H ), LDHA bands are quantified in ( I ), PKM2 bands are quantified in ( J ), and pPKM2 (Tyr105) bands are quantified in ( K ). N = 3 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired. ( L – M ) qPCR analysis reveals that miR-181a/b expression does not change in the NR of Pde6β H620Q miR-181a/b-1 −/− in comparison to Pde6β H620Q miR-181a/b-1 +/+ at ( L ) PW6, and M PW10. N = 4 mice/genotype. Data are presented as mean ± SEM. Student’s t-test, unpaired

    Article Snippet: The primary antibodies used were rabbit anti-PKM2 polyclonal antibody (1:1000; Cell signaling, 3198), rabbit anti-pPKM2 (phosphorylation Tyr105) polyclonal antibody (1:1000; Cell signaling, 3827), rabbit anti-LDHA polyclonal antibody (1:1000; Thermo Fisher Scientific, PA5-23036), and mouse anti-β-actin monoclonal antibody (1:2000; Cell signaling, 8H10D10).

    Techniques: Expressing, Western Blot, Comparison

    The LDHA expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: The LDHA expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Expressing, Quantitative RT-PCR, Staining

    Enrichment analysis of LDHA-related genes in EC. (A) LDHA-related genes in TCGA-UCEC cohort detected by the LinkedOmics database. The top 50 co-expression genes positively (B) and negatively (C) associated with LDHA in the TCGA-UCEC cohort. (D–F) Enrichment analysis of (GO) terms for LDHA-related genes. (G) Enrichment analysis of KEGG terms for LDHA-related genes.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Enrichment analysis of LDHA-related genes in EC. (A) LDHA-related genes in TCGA-UCEC cohort detected by the LinkedOmics database. The top 50 co-expression genes positively (B) and negatively (C) associated with LDHA in the TCGA-UCEC cohort. (D–F) Enrichment analysis of (GO) terms for LDHA-related genes. (G) Enrichment analysis of KEGG terms for LDHA-related genes.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Expressing

    Influence of LDHA knockdown on EC functions. (A) LDHA protein level after LDHA knockdown detected by Western Blot. (B, C) Cell proliferation after LDHA knockdown detected by CCK-8 assay. (D, E) Cell proliferation after LDHA knockdown detected by cell clone formation assay. (F, G) . Cell apoptosis after LDHA knockdown detected by flow cytometry. (H, I) Cell migration after LDHA knockdown was detected by the Transwell assay. (J, K) Cell invasion after LDHA knockdown was detected by the Transwell assay. * P <0.05.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Influence of LDHA knockdown on EC functions. (A) LDHA protein level after LDHA knockdown detected by Western Blot. (B, C) Cell proliferation after LDHA knockdown detected by CCK-8 assay. (D, E) Cell proliferation after LDHA knockdown detected by cell clone formation assay. (F, G) . Cell apoptosis after LDHA knockdown detected by flow cytometry. (H, I) Cell migration after LDHA knockdown was detected by the Transwell assay. (J, K) Cell invasion after LDHA knockdown was detected by the Transwell assay. * P <0.05.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Knockdown, Western Blot, CCK-8 Assay, Tube Formation Assay, Flow Cytometry, Migration, Transwell Assay

    Associations between LDHA and tumor immune infiltrating cells. (A) Correlation of LDHA to stromal cells and immune cells calculated by the ESTIMATE method. (B) Relationship between LDHA expression and infiltration levels of immune cells. (C) Enrichment scores of immune cells in the high LDHA group and low LDHA group. (D) Infiltration levels of immune cells in WT LDHA group and mutated LDHA group. (E) The survival curves of patients with different combinations of LDHA and immune cells. WT, wild type. * P <0.05, ** P <0.01, *** P <0.001.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Associations between LDHA and tumor immune infiltrating cells. (A) Correlation of LDHA to stromal cells and immune cells calculated by the ESTIMATE method. (B) Relationship between LDHA expression and infiltration levels of immune cells. (C) Enrichment scores of immune cells in the high LDHA group and low LDHA group. (D) Infiltration levels of immune cells in WT LDHA group and mutated LDHA group. (E) The survival curves of patients with different combinations of LDHA and immune cells. WT, wild type. * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Expressing

    Associations between LDHA and gene markers of immune cells in TIMER, GEPIA, and TCGA database.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Associations between LDHA and gene markers of immune cells in TIMER, GEPIA, and TCGA database.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Marker

    Associations between LDHA expression and ferroptosis-related genes in EC. (A) Connection of LDHA to ferroptosis-related genes in GSE106191 and TCGA-UCEC cohort. (B) Connection of LDHA to FANCD2 and TFRC in TCGA-UCEC cohort. (C) Connection of LDHA to FANCD2 and TFRC in GSE106191. (D) The differential expression of ferroptosis-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F, G) The Kaplan–Meier curve of hub genes. (H, I) The changes of ferroptosis-related genes after LDHA knockdown in EC cells. * P <0.05, ** P <0.01, *** P <0.001.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Associations between LDHA expression and ferroptosis-related genes in EC. (A) Connection of LDHA to ferroptosis-related genes in GSE106191 and TCGA-UCEC cohort. (B) Connection of LDHA to FANCD2 and TFRC in TCGA-UCEC cohort. (C) Connection of LDHA to FANCD2 and TFRC in GSE106191. (D) The differential expression of ferroptosis-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F, G) The Kaplan–Meier curve of hub genes. (H, I) The changes of ferroptosis-related genes after LDHA knockdown in EC cells. * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Expressing, Quantitative Proteomics, Knockdown

    Associations between LDHA expression and m6A-related genes in EC. (A) Connection of LDHA to m6A-related genes in GSE106191 and TCGA-UCEC cohort. (B) Connection of LDHA to ALKBH5 and HNRNPC in TCGA-UCEC cohort. (C) Connection of LDHA to ALKBH5 and HNRNPC in GSE106191. (D) The differential expression of m6A-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F) The Kaplan–Meier curve of hub genes. * P <0.05, *** P <0.001.

    Journal: Frontiers in Oncology

    Article Title: Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer

    doi: 10.3389/fonc.2024.1458344

    Figure Lengend Snippet: Associations between LDHA expression and m6A-related genes in EC. (A) Connection of LDHA to m6A-related genes in GSE106191 and TCGA-UCEC cohort. (B) Connection of LDHA to ALKBH5 and HNRNPC in TCGA-UCEC cohort. (C) Connection of LDHA to ALKBH5 and HNRNPC in GSE106191. (D) The differential expression of m6A-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F) The Kaplan–Meier curve of hub genes. * P <0.05, *** P <0.001.

    Article Snippet: The primary antibody was Rabbit polyclonal Anti-LDHA antibody (1:1000, #PB10075, Boster, China) or β-actin mouse monoclonal antibody (1:2000, #AF2811, Beyotime, China).

    Techniques: Expressing, Quantitative Proteomics